Grobogan Regency, Central Java, is characterized by karst landscapes, particularly in the Sukolilo Karst Landscape Area (Kawasan Bentang Alam Karst—KBAK Sukolilo), which plays a crucial role in regional water availability. Although the northern and western parts of the regency have the potential to store groundwater due to karstification, the southern regions often face water scarcity and prolonged droughts. This study aims to (1) identify the spatial distribution of springs in KBAK Sukolilo, and (2) analyze water availability across the karst area. A quantitative research approach was employed, focusing on 43 springs distributed across six subdistricts: Klambu, Brati, Grobogan, Tawangharjo, Wirosari, and Ngaringan. Data collection involved field observations, interviews, and documentation, while spatial and environmental analyses were conducted using Geographic Information Systems (GIS). The findings reveal an uneven and dynamic distribution of springs, with some springs recorded in 2018 no longer active or traceable in 2024 due to land cover changes and diminished discharge. Based on study, it was seen that the water demand in three sub-districts exceeded the water availability. Water balance analysis indicates that Brati, Grobogan, and Ngaringan are categorized as water-deficit areas, whereas Klambu, Tawangharjo, and Wirosari experience a water surplus. These results highlight the urgent need for integrated water resource management in karst environments, particularly in addressing regional disparities in water availability.