The Jaintiapur-Jaflong region, strategically positioned between the subsiding Surma Basin to the south and the uplifting Shillong Massif to the north, presents a unique geological setting. This study employed geological clinometers and other field methods to ascertain the geological characteristics of the area. The regional strike was determined to be N66˚W, with a dip direction of S24˚W and a dip angle of 42.25˚. Through extensive field investigations, including geological mapping, stratigraphic logging, rock sampling, fossil analysis, and structural analysis, complemented by Global Positioning System (GPS), photography, remote sensing, and Geographic Information System (GIS) technologies, seven lithostratigraphic units were identified. These include the variegated color sandstone, mottled clay, yellowish to reddish-grey sandstone, sandy shale with intercalated silty shale, pinkish sandstone, bluish to blackish-grey shale, and limestone units, corresponding sequentially to Dupi Tila, Girujan Clay, Tipam Sandstone, Surma Group, Jenum Shale Fm, Kopili Shale, and Sylhet Limestone Fm, respectively. Five critical geological contact boundaries were delineated, with notable boundaries identified at the Dupigaon-Sari River Section, the Lalakhal-Tetulghat Section, the Nayagang-Gourishankar Section, and between the Barail and Jaintia groups at the Tamabil-Jaflong Highway Road Cut Section. These findings elucidate the geological contacts and stratigraphic units, providing significant implications for paleoenvironmental reconstruction, resource potential assessment, and stratigraphic correlation, thus enhancing the understanding of regional geological history and laying a foundation for future research endeavors.